This long-eared bat is restricted to forests in southwestern Western Australia. Genetic and morphological comparisons showed that the western population once treated as the same species as eastern long-eared bats is distinct.
The original description reports a higher post-nasal mound behind the noseleaf and relatively longer ears in N. holtorum than in the related N. daedalus.
Its ecology is not well known, but it seems to favor forests with a shrubby understory and many potential roosts in old tree hollows.
An extinction-risk assessment has not been added yet.